Vitrogen Coating Flasks

1) Add 1 ml Vitrogen to 44 ml of cold 1X PBS.
2) Add 3 ml dilution for every 25 cm2 surface area of flask.
3) Tilt flask to make sure entire surface is coated
4) Store in air tight container, on even surface at 4oC for a minimum of overnight and a maximum of three weeks.
5) Only open airtight container under sterile hood.

Before plating cells into a Vitrogen coated flask
1) Aspirate Vitrogen mixture
2) Wash once with 10 ml DMEM/F12
3) Add warmed H14 medium.

Vitrogen Coating Flasks/SubPage (last edited 2012-07-06 23:18:19 by c-98-248-250-205)